Coding

Part:BBa_K3514001:Design

Designed by: Oona Jung   Group: iGEM20_BOKU-Vienna   (2020-10-22)


Protein Exo of the Lambda Red System, degrades dsDNA into ssDNA, leaves 3' ends


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Optimized to be compatible for the igem registry. Silent mutations: bp 31: g->c ; bp 289: a->g ; bp 221: c->a ;

Source

Sequence provided by KEGG(entry:2703522) database

References

NCBI Reference Sequence: NP_040616.1

Christopher R. T. Hillyar, Genetic recombination in bacteriophage lambda, Bioscience Horizons: The International Journal of Student Research, Volume 5, 2012, hzs001, https://doi.org/10.1093/biohorizons/hzs001

Paul G. Mitsis, Jae G. Kwagh, Characterization of the interaction of lambda exonuclease with the ends of DNA, Nucleic Acids Research, Volume 27, Issue 15, 1 August 1999, Pages 3057–3063, https://doi.org/10.1093/nar/27.15.3057